rabbit anti il 1r1 antibody  (Bioss)

Journal: Cureus

Article Title: Melittin Alleviates Oxidative Stress Injury in Schwann Cells by Targeting Interleukin-1 Receptor Type 1 to Downregulate Nuclear Factor Kappa B-Mediated Inflammatory Response In Vitro

doi: 10.7759/cureus.65721

Figure Lengend Snippet: (A) and (B): RT-qPCR analysis of reactive mRNA of IL-1β and TNF-α expression in hypoxic SCs and the intervention effect of melittin. (C) Immunofluorescence assays after staining with anti-Fn and anti-IL-1R1 antibodies (scale bars = 50 μm). ****p < 0.0001 versus the control group. Data are presented as mean ± standard deviation. SCs: Schwann cells; RT-qPCR: Quantitative reverse transcription-polymerase chain reaction; IL-1R1: Interleukin-1 receptor type 1; TNF-α: Tumor necrosis factor-alpha; DAPI: 4′,6-diamidino-2-phenylindole.

Article Snippet: Briefly, cells cultured on coverslips were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.05% Triton X-100 for 10 min. Non-specific binding sites were blocked with 5% goat serum for 1 h. Subsequently, the cells were incubated with a rabbit anti-rat IL-1R1 monoclonal antibody (AF7212, Beyotime, Jiangsu, China) at room temperature for 1.5 h. After washing three times with PBS, the cells were incubated with FITC-conjugated goat anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature.

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Control, Standard Deviation, Reverse Transcription, Polymerase Chain Reaction

Journal: Cureus

Article Title: Melittin Alleviates Oxidative Stress Injury in Schwann Cells by Targeting Interleukin-1 Receptor Type 1 to Downregulate Nuclear Factor Kappa B-Mediated Inflammatory Response In Vitro

doi: 10.7759/cureus.65721

Figure Lengend Snippet: (A) RT-qPCR results showing the expression levels of IL-1R1 and IL-1β. (B) Transmission electron microscope images of ultrastructural alterations in CoCl 2 -induced SCs for each group (Left: ×2500, Right: ×7000), with the red arrows indicating mitochondria. (C) Western blot of C-JUN and GDNF protein expression for quantitative analyses. (D) and (E) Western blot and RT-qPCR results showing the expression levels of VEGF, HIF-1α, IL-1R1, ENO1, AR, SOD, NGF, and iNOS. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control and other groups. Data are presented as mean ± standard deviation. SCs: Schwann cells; GDNF: Glial cell line-derived neurotrophic factor; RT-qPCR: Quantitative reverse transcription-polymerase chain reaction; VEGF: Vascular endothelial growth factor; HIF-1α: Hypoxia-inducible factor 1-alpha; IL-1R1: Interleukin-1 receptor type 1; ENO1: Enolase 1; AR: Aldose reductase; SOD: Superoxide dismutase; NGF: Nerve growth factor; iNOS: Inducible nitric oxide synthase.

Article Snippet: Briefly, cells cultured on coverslips were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.05% Triton X-100 for 10 min. Non-specific binding sites were blocked with 5% goat serum for 1 h. Subsequently, the cells were incubated with a rabbit anti-rat IL-1R1 monoclonal antibody (AF7212, Beyotime, Jiangsu, China) at room temperature for 1.5 h. After washing three times with PBS, the cells were incubated with FITC-conjugated goat anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature.

Techniques: Quantitative RT-PCR, Expressing, Transmission Assay, Microscopy, Western Blot, Control, Standard Deviation, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction

Journal: Cureus

Article Title: Melittin Alleviates Oxidative Stress Injury in Schwann Cells by Targeting Interleukin-1 Receptor Type 1 to Downregulate Nuclear Factor Kappa B-Mediated Inflammatory Response In Vitro

doi: 10.7759/cureus.65721

Figure Lengend Snippet: (A-E) RT-qPCR results showing mRNA expressions of IKK, IKB-α, p65, p60, and IRAK1. (F) Western blot results of protein expression of IKK, IKB-α, p65, p60, and IRAK1. (G-K) Quantitative analyses of IKK, IKB-α, p65, p60, and IRAK1 protein expression. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control and other groups. Data are presented as mean ± standard deviation. RT-qPCR: Quantitative reverse transcription-polymerase chain reaction; IL-1R1: Interleukin-1 receptor type 1; NF-κB: Nuclear factor kappa B.

Article Snippet: Briefly, cells cultured on coverslips were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.05% Triton X-100 for 10 min. Non-specific binding sites were blocked with 5% goat serum for 1 h. Subsequently, the cells were incubated with a rabbit anti-rat IL-1R1 monoclonal antibody (AF7212, Beyotime, Jiangsu, China) at room temperature for 1.5 h. After washing three times with PBS, the cells were incubated with FITC-conjugated goat anti-rabbit IgG (#7074, Cell Signaling) for 2 h at room temperature.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control, Standard Deviation, Reverse Transcription, Polymerase Chain Reaction

Journal: The American Journal of Pathology

Article Title: Fibroblasts Promote Inflammation and Pain via IL-1α Induction of the Monocyte Chemoattractant Chemokine (C-C Motif) Ligand 2

doi: 10.1016/j.ajpath.2017.11.007

Figure Lengend Snippet: Synovial fluid and tissues isolated from patients undergoing revision total knee arthroplasty (TKA) are characterized by dramatic tissue remodeling and chronic inflammation. A: Representative images of Masson trichrome and IL-1R1 (green [fluorescein isothiocyanate (FITC)])/vimentin (red [tetrarhodamine isothiocyanate (TRITC)]) stained infrapatellar fat pad from patients undergoing primary TKA and patients undergoing revision TKA. There is a significant increase in collagen and IL-1R1 expression in revision TKA tissue compared with primary TKA tissue, and IL-1R1 is predominantly expressed on elongated bipolar mesenchymal cells in revision TKA tissue. Images acquired on a Nikon inverted microscope and a Nikon A1R point scanning confocal microscope, respectively. B: Venn diagram demonstrating change in protein expression in synovial fluid, infrapatellar fat pad, and synovial membrane isolated from patients undergoing revision TKA compared with patients undergoing primary TKA. Protein expression was quantified using human V-Plex electrochemiluminescence detection kits from MesoScaleDiscovery. Markers in black text are significantly elevated in revision TKA, markers in blue text are not significantly different or undetectable, and markers in red text are significantly decreased in revision TKA. Significance was taken as P < 0.05. C: Representative images of vimentin [green (FITC)]– and IL-6 [red (TRITC)]–stained infrapatellar fat pad from patients undergoing revision TKA. There are a significant number of vimentin and IL-6 dual-positive fibroblasts in post-TKA infrapatellar fat pad. Images acquired on a Nikon A1R point scanning confocal microscope. Original magnification, ×20 ( A and C ); ×40 ( inset ). CRP, C-reactive protein; FGF, fibroblast growth factor; Flt, Fms-like tyrosine kinase 1; GM-CSF, granulocyte-macrophage colony-stimulating factor; ICAM, intercellular adhesion molecule; IFN, interferon; IP-10, interferon gamma-induced protein 10; PIGF, placental growth factor; SAA, serum amyloid A; Tie, tyrosine kinase; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule; VEGF, vascular endothelial growth factor.

Article Snippet: Primary antibody incubation was performed overnight at 4°C using rabbit polyclonal primary antibody against IL-1R1 (Ab106278; Abcam, Cambridge, UK).

Techniques: Isolation, Staining, Expressing, Inverted Microscopy, Microscopy, Membrane, Electrochemiluminescence